Use of Luminescent Leptospira interrogans for Enumeration in Biological Assays
Gerald L Murray, Amy M King, Amporn Srikram, Rasana W Sermswan, Ben Adler
JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2010
Rapid and reliable in vitro methods for the detection of pathogenic leptospires, such as Leptospira interrogans, are lacking. The present study investigated the use of luminescence to replace the existing enumeration techniques. Transposon TnSC189 was modified to incorporate the luxCDABE cassette from Photorhabdus luminescens and was used to construct luminescent Leptospira spp. There was a linear relationship between luminescence and cell number, with the theoretical detection limit being less than 10(4) leptospires. A comparison of enumeration by a standard method (counting by dark-field microscopy) and enumeration by luminescence was conducted with luminescent L. interrogans. There was a ..View full abstract
This work was supported by the National Health and Medical Research Council (NHMRC) of Australia and the Australian Research Council. G. L. M. is supported by an NHMRC Peter Doherty Fellowship.