Journal article

Generally applicable transcriptome-wide analysis of translation using anota2seq

Christian Oertlin, Julie Lorent, Carl Murie, Luc Furic, Ivan Topisirovic, Ola Larsson

Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2019

Abstract

mRNA translation plays an evolutionarily conserved role in homeostasis and when dysregulated contributes to various disorders including metabolic and neurological diseases and cancer. Notwithstanding that optimal and universally applicable methods are critical for understanding the complex role of translational control under physiological and pathological conditions, approaches to analyze translatomes are largely underdeveloped. To address this, we developed the anota2seq algorithm which outperforms current methods for statistical identification of changes in translation. Notably, in contrast to available analytical methods, anota2seq also allows specific identification of an underappreciate..

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Grants

Awarded by Canadian Institutes for Health Research


Awarded by National Institutes of Health


Awarded by Joint Canada-Israel Health Research Program (JCIHRP)


Awarded by Department of Health and Human Services acting through theVictorian Cancer Agency


Awarded by National Health and Medical Research Council (NHMRC)


Awarded by NATIONAL CANCER INSTITUTE


Funding Acknowledgements

Swedish Research Council; Swedish Cancer Society; Cancer Society in Stockholm; Wallenberg Academy Fellows Program; STRATCAN grants (to O.L.); Canadian Institutes for Health Research [MOP-363027]; National Institutes of Health [R01 CA 202021-01-A1 to I.T.] who is a Junior 2 Research Scholar of the Fonds de Recherche du Quebec Sante (FRQ-S); Joint Canada-Israel Health Research Program (JCIHRP) [108589-001 to I.T. and O.L.]; Department of Health and Human Services acting through theVictorian Cancer Agency [MCRF16007 to L.F.]; National Health and Medical Research Council (NHMRC) [APP1141339 to O.L., I.T. and L.F.] Funding for open access charge: Vetenskapsradet.