Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

Kinga M Sulyok; Zsuzsa Kreizinger; Katinka Beko; Barbara Forro; Szilvia Marton; Krisztian Banyai; Salvatore Catania; Christine Ellis; Janet Bradbury; Olusola M Olaogun; Aron B Kovacs; Tibor Cserep; Miklos Gyuranecz

Journal article

Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

Kinga M Sulyok, Zsuzsa Kreizinger, Katinka Beko, Barbara Forro, Szilvia Marton, Krisztian Banyai, Salvatore Catania, Christine Ellis, Janet Bradbury, Olusola M Olaogun, Aron B Kovacs, Tibor Cserep, Miklos Gyuranecz

Journal of Clinical Microbiology | American Society for Microbiology | Published : 2019

DOI: 10.1128/JCM.01084-18

Abstract

Mycoplasma gallisepticum is among the most economically significant mycoplasmas causing production losses in poultry. Seven melt-curve and agarose gel-based mismatch amplification mutation assays (MAMAs) and one PCR are provided in the present study to distinguish the M. gallisepticum vaccine strains and field isolates based on mutations in the crmA, gapA, lpd, plpA, potC, glpK, and hlp2 genes. A total of 239 samples (M. gallisepticum vaccine and type strains, pure cultures, and clinical samples) originating from 16 countries and from at least eight avian species were submitted to the presented assays for validation or in blind tests. A comparison of the data from 126 samples (including sequ..

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University of Melbourne Researchers

Martins Olaogun Author Veterinary Biosciences

Grants

Awarded by Lendulet program of the Hungarian Academy of Sciences

Awarded by National Research, Development and Innovation Fund, Hungary

Awarded by Bolyai+ Fellowship of the New National Excellence Program of the Ministry of Human Capacities

Funding Acknowledgements

This work was supported by the Lendulet program (grant LP2012-22) of the Hungarian Academy of Sciences, and the K119594, FK_17 (124019), and KKP_19 (129751) grants of the National Research, Development and Innovation Fund, Hungary. Z.K., S.M., and M.G. were supported by the Bolyai Janos Fellowship of the Hungarian Academy of Sciences. M.G. was supported by the Bolyai+ Fellowship (UNKP-18-4) of the New National Excellence Program of the Ministry of Human Capacities. The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.