Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo

J Easterbrook; S Rybtsov; S Gordon-Keylock; A Ivanovs; S Taoudi; RA Anderson; A Medvinsky

Journal article

Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo

J Easterbrook, S Rybtsov, S Gordon-Keylock, A Ivanovs, S Taoudi, RA Anderson, A Medvinsky

Stem Cell Reports | CELL PRESS | Published : 2019

DOI: 10.1016/j.stemcr.2019.03.003

Abstract

Definitive hematopoietic stem cells (HSCs)first emerge in the aorta-gonad-mesonephros (AGM)region in both mice and humans. An ex vivo culture approach has enabled recapitulation and analysis of murine HSC development. Knowledge of early human HSC development is hampered by scarcity of tissue: analysis of both CFU-C and HSC development in the human embryo is limited. Here, we characterized the spatial distribution and temporal kinetics of CFU-C development within early human embryonic tissues. We then sought to adapt the murine ex vivo culture system to recapitulate human HSC development. We show robust expansion of CFU-Cs and maintenance, but no significant expansion, of human HSCs in cultur..

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University of Melbourne Researchers

Samir Taoudi Author

Grants

Awarded by UK Research and Innovation

Funding Acknowledgements

We thank the patients for donating tissue and A. Saunderson, J. Creiger, and N. Forson for gaining consent from patients and collecting specimens. We thank J. Verth, C. Manson, and their staff for animal maintenance. This work was supported by the Wellcome Trust (grant 100468/Z/12/Z, J.E.) and the MRC (grant G0901577, A.M.).