Journal article

The Microbiome in Paediatric Crohn's Disease-A Longitudinal, Prospective, Single-Centre Study

Shivani Kansal, Anthony G Catto-Smith, Karen Boniface, Sarah Thomas, Donald J Cameron, Mark Oliver, George Alex, Carl D Kirkwood, Josef Wagner

Journal of Crohn's and Colitis | OXFORD UNIV PRESS | Published : 2019

Abstract

BACKGROUND AND AIMS: The gut mucosa is the principal site where Crohn's disease [CD] inflammation occurs. Limited information is available about the gut mucosal microbiome during CD relapse and remission. The aim of our study was to characterize specific changes in the gut microbiome during relapse and remission in a large single-centre paediatric CD cohort. METHODS: We analysed the microbiome of 345 biopsies from 204 patients, including 88 CD first diagnosis [CDFD] patients, 38 relapse [CDRL] patients, 12 remission [CDRM] patients, and 66 controls. Species identification was conducted using oligotyping in combination with ARB/SILVA taxonomic annotation. RESULTS: We observed 45 bacteria to d..

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Grants

Awarded by NHMRC Research Gellowships


Funding Acknowledgements

This work was supported by research grants from the Murdoch Children's Research Institute, the Australian National Health and Medical Research Council [NHMRC], the Victoria State scientific infrastructure scheme, the CASS foundation, and the Broad Foundation. CK was supported by NHMRC Research Gellowships [607347, 1078541].