Journal article

Cisplatin- and cyclophosphamide-induced primordial follicle depletion is caused by direct damage to oocytes

QN Nguyen, N Zerafa, SH Liew, JK Findlay, M Hickey, KJ Hutt

Molecular Human Reproduction | OXFORD UNIV PRESS | Published : 2019

Abstract

It is well established that DNA-damaging chemotherapies can cause infertility and ovarian endocrine failure by depleting the ovarian reserve of primordial follicles. Currently, no effective pharmacological therapies exist for the preservation of long-term fertility and ovarian function in female cancer patients, due to a limited understanding of the mechanisms of chemotherapy-induced follicle depletion. This study investigated the cellular targets, molecular mechanisms, and temporal course of ovarian reserve depletion following treatment with commonly used chemotherapeutic drugs. Adult female C57BL/6 mice were injected i.p. with saline, cisplatin (5mg/kg), or cyclophosphamide (300mg/kg); ova..

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Grants

Awarded by National Health and Medical Research Council Australia


Awarded by National Breast Cancer Foundation


Funding Acknowledgements

This work was supported by fellowships and grants from the National Health and Medical Research Council Australia (Project Grant #1007027, Program Grant #1016701, and Fellowship KJH #1050130); National Breast Cancer Foundation (#NC14-001). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.