Journal article

Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment

Rocio G Urdinguio, Virginia Lopez, Gustavo F Bayon, Rafael Diaz de la Guardia, Marta I Sierra, Estela Garcia-Torano, Raul F Perez, Maria G Garcia, Antonella Carella, Patricia C Pruneda, Cristina Prieto, Marija Dmitrijeva, Pablo Santamarina, Thalia Belmonte, Cristina Mangas, Elena Diaconu, Cecilia Ferrero, Juan Ramon Tejedor, Juan Luis Fernandez-Morera, Cristina Bravo Show all

NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2019

Abstract

Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions pres..

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Grants

Awarded by Plan Nacional de I+D+I


Awarded by Plan Nacional de I+D+I 2008-2011/FEDER


Awarded by Ministry of Economy and Competitiveness Juan de la Cierva postdoctoral fellowships


Awarded by Asturias Regional Government


Awarded by Gobierno del Principado de Asturias, PCTI-Plan de Ciencia, Tecnologia e Innovacion


Awarded by Deutsche Forschungsgemeinschaft (DFG)


Awarded by European Research Council


Awarded by Spanish Ministry of Economy and Competitiveness


Awarded by ISCIII


Awarded by Generalitat de Catalunya


Funding Acknowledgements

Plan Nacional de I+D+I co-funding FEDER [PI15/00892 and PI18/01527 to M.F.F. and A.F.F.; PI16/01318 and PI14/01244 to C.L.]; ISCIII-Subdireccion General de Evaluaciccion y Fomento de la Investigacion, and Plan Nacional de I+D+I 2008-2011/FEDER [CP11/00131 to A.F.F.]; IUOPA (to G.F.B. and M.I.S.); Fundacion Cientifica de la AECC (to R.G.U.); Ministry of Economy and Competitiveness Juan de la Cierva postdoctoral fellowships [FJCI-2015-26965 to J.R.T., IJCI-2015-23316 to V.L.]; Fundacion Ramon Areces (to M.F.F); FICYT (to E.G.T., M.G.G., A.C.); Asturias Regional Government [GRUPIN14-052 to M.F.F.]; Gobierno del Principado de Asturias, PCTI-Plan de Ciencia, Tecnologia e Innovacion co-funding Fondos FEDER (grant number IDI/2018/146 to M.F.F. and IDI/2018/144 to C.L.); Deutsche Forschungsgemeinschaft (DFG) [SFB960 to A.V.G., R.D.]; European Research Council [CoG-2014-646903]; Spanish Ministry of Economy and Competitiveness [SAF-SAF2013-43065 to P.M.]; Asociacion Espanola Contra el Cancer [AECC-CI-2015]; FERO Foundation, and the ISCIII [PI14-01191 to C.B.]; P.M. acknowledges financial support from The Obra Social La Caixa-Fundacio Josep Carreras and The Generalitat de Catalunya (SGR330). P.M. an investigator from the Spanish Cell Therapy cooperative network (TERCEL). The IUOPA is supported by the Obra Social Liberbank-Cajastur, Spain. Funding for open access charge: Plan Nacional de I+D+I co-funding FEDER [PI18/01527].