Journal article

Molecular Evidence of Drug-Resistant Tuberculosis in the Balimo Region of Papua New Guinea

Tanya Diefenbach-Elstob, Vanina Guernier, Graham Burgess, Daniel Pelowa, Robert Dowi, Bisato Gula, Munish Puri, William Pomat, Emma McBryde, David Plummer, Catherine Rush, Jeffrey Warner

TROPICAL MEDICINE AND INFECTIOUS DISEASE | MDPI | Published : 2019

Abstract

Papua New Guinea (PNG) has a high burden of tuberculosis (TB), including drug-resistant TB (DR-TB). DR-TB has been identified in patients in Western Province, although there has been limited study outside the provincial capital of Daru. This study focuses on the Balimo region of Western Province, aiming to identify the proportion of DR-TB, and characterise Mycobacterium tuberculosis (MTB) drug resistance-associated gene mutations. Sputum samples were investigated for MTB infection using published molecular methods. DNA from MTB-positive samples was amplified and sequenced, targeting the rpoB and katG genes to identify mutations associated with rifampicin and isoniazid resistance respectively..

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Grants

Funding Acknowledgements

This research was funded by two unrestricted grants from the Queensland Government Department of Science, Information Technology and Innovation (DSITI) through the Australian Institute of Tropical Health and Medicine (AITHM). The grant recipients were (1) Emma McBryde and JeffreyWarner; and (2) Catherine Rush and Jeffrey Warner.