Journal article
A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka
A Herpin, C Schmidt, S Kneitz, C Gobé, M Regensburger, A Le Cam, J Montfort, MC Adolfi, C Lillesaar, D Wilhelm, M Kraeussling, B Mourot, B Porcon, M Pannetier, E Pailhoux, L Ettwiller, D Dolle, Y Guiguen, M Schartl
Plos Biology | PUBLIC LIBRARY SCIENCE | Published : 2019
Abstract
Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1—acting as master sex-determining gene—has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 30 UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them respons..
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Awarded by Deutsche Forschungsgemeinschaft
Awarded by Agence Nationale de la Recherche (ANR)
Funding Acknowledgements
This work was supported by the Deutsche Forschungsgemeinschaft by a grant (SCHA 408/12-1; HE 7135/2-1) to AH and MS as well as Credits Incitatifs Phase 2015/Emergence to AH. AH was additionally funded by the project AquaCRISPR (ANR-16-COFA-0004-01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.