Journal article
Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation
KL Inder, JE Ruelcke, L Petelin, H Moon, E Choi, J Rae, A Blumenthal, D Hutmacher, NA Saunders, JL Stow, RG Parton, MM Hill
Journal of Extracellular Vesicles | TAYLOR & FRANCIS LTD | Published : 2014
DOI: 10.3402/jev.v3.23784
Abstract
Background: Tumour-derived extracellular vesicles (EVs) play a role in tumour progression; however, the spectrum of molecular mechanisms regulating EV secretion and cargo selection remain to be fully elucidated. We have reported that cavin-1 expression in prostate cancer PC3 cells reduced the abundance of a subset of EV proteins, concomitant with reduced xenograft tumour growth and metastasis. Methods: We examined the functional outcomes and mechanisms of cavin-1 expression on PC3-derived EVs (PC3-EVs). Results: PC3-EVs were internalized by osteoclast precursor RAW264.7 cells and primary human osteoblasts (hOBs) in vitro, stimulating osteoclastogenesis 37-fold and hOB proliferation 1.5-fold,..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This project was supported by the Prostate Cancer Foundation Australia. MMH was supported by a Career Development Fellowship from the National Health and Medical Research Council (NHMRC) of Australia (APP 569512), RGP was supported by NHMRC fellowships and grants (grant numbers 569542, 1045092 and 1037320). The authors acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.