Journal article

A role for Rab11 in the homeostasis of the endosome-lysosomal pathway

Khalisah L Zulkefli, Fiona J Houghton, Prajakta Gosavi, Paul A Gleeson

EXPERIMENTAL CELL RESEARCH | ELSEVIER INC | Published : 2019

Abstract

The small GTPases Rab11a and 11b are key regulators of membrane transport, localised to the recycling endosomes and also early endosomes. The function of Rab11 within the recycling pathway has been well defined, however, the role of Rab11 at the early endosomes remains poorly characterised. Here, we have generated HeLa cell lines devoid of either Rab11a or Rab11b using CRISPR/Cas9 to functionally dissect the roles of these two Rab11 family members in recycling and in the endosomal-lysosomal system. Both Rab11a and Rab11b contribute to the dynamics of tubulation arising from recycling endosomes whereas Rab11a has the major role in recycling of transferrin receptor. Deletion of either Rab11a o..

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Grants

Awarded by Australian Research Council


Funding Acknowledgements

This work was supported by funding from the Australian Research Council (DP130103207). KLZ is supported by University of Melbourne International Graduate Scholarship. We acknowledge the Advanced Microscopy Facility at the Bio21 Molecular Science and Biotechnology Institute, for assistance with electron microsopy. Confocal Microscopy was performed within the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne.