Journal article

A novel method for screening viral interferon-resistance genes.

Daniel TW Clarke, Aaron T Irving, Eleanore H Lambley, Elizabeth Payne, Nigel AJ McMillan

J Interferon Cytokine Res | Published : 2004

Abstract

Many viruses have evolved mechanisms to antagonize the interferon (IFN) system, targeting all the major components involved in receptor binding and signaling. Although a number of these vital proteins are homologous to cellular proteins involved in IFN downregulation (e.g., viral IFN regulatory factors [vIRFs]), many share little resemblance to known proteins. To determine the IFN-blocking properties of these proteins, functional assays are required. Here, we present a new and rapid functional screening method, based on the 2fTGH cell line, which is able to determine viral gene products that inhibit the IFN-alpha/Jak-Stat signaling pathway. Expression cloning of viral IFN-blocking genes into..

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University of Melbourne Researchers