Journal article

Human LC3 and GABARAP subfamily members achieve functional specificity via specific structural modulations

Nidhi Jatana, David B Ascher, Douglas E Pires, Rajesh S Gokhale, Lipi Thukral

Autophagy | TAYLOR & FRANCIS INC | Published : 2020

Abstract

Autophagy is a conserved adaptive cellular pathway essential to maintain a variety of physiological functions. Core components of this machinery are the six human Atg8 orthologs that initiate formation of appropriate protein complexes. While these proteins are routinely used as indicators of autophagic flux, it is presently not possible to discern their individual biological functions due to our inability to predict specific binding partners. In our attempts towards determining downstream effector functions, we developed a computational pipeline to define structural determinants of human Atg8 family members that dictate functional diversity. We found a clear evolutionary separation between h..

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Grants

Awarded by Jack Brockhoff Foundation


Awarded by Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)


Awarded by INSPIRE Faculty fellowship, Department of Science and Technology, Government of India


Awarded by NHMRC CJ Martin Fellowship


Awarded by Science and Engineering Research Board


Funding Acknowledgements

This work was supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; Jack Brockhoff Foundation [JBF 4186, 2016]; Newton Fund RCUK-CONFAP Grant awarded by the Medical Research Council (MRC) and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [MR/M026302/1]; IRR/FIOCRUZ Minas; Department of Biotechnology, Ministry of Science and Technology (IN); INSPIRE Faculty fellowship, Department of Science and Technology, Government of India (IN) [LSBM-45]; NHMRC CJ Martin Fellowship [APP1072476]; Science and Engineering Research Board [PDF/2017/000905].