Lipopolysaccharide reduces gonadotrophin-releasing hormone (GnRH) gene expression: role of RFamide-related peptide-3 and kisspeptin
Chooi Yeng Lee, ShengYun Li, Xiao Feng Li, Daniel AE Stalker, Claire Cooke, Bei Shao, Haluk Kelestimur, Belinda A Henry, Gregory Conductier, Kevin T O'Byrne, Iain J Clarke
Reproduction, Fertility and Development | CSIRO PUBLISHING | Published : 2019
RFamide-related peptide (RFRP)-3 reduces luteinising hormone (LH) secretion in rodents. Stress has been shown to upregulate the expression of the RFRP gene (Rfrp) with a concomitant reduction in LH secretion, but an effect on expression of the gonadotrophin-releasing hormone (GnRH) gene (Gnrh1) has not been shown. We hypothesised that lipopolysaccharide (LPS)-induced stress affects expression of Rfrp, the gene for kisspeptin (Kiss1) and/or Gnrh1, leading to suppression of LH levels in rats. Intracerebroventricular injections of RFRP-3 (0.1, 1, 5 nmol) or i.v. LPS (15μgkg-1) reduced LH levels. Doses of 1 and 5 nmol RFRP-3 were then administered to analyse gene expression by in situ hybridisat..View full abstract
This work was supported by the Biotechnology and Biological Sciences Research Council, UK, The National Health and Medical Research Council of Australia and Monash University Malaysia Outside Study Program.