Preterm Lung Exhibits Distinct Spatiotemporal Proteome Expression at Initiation of Lung Injury
Prue M Pereira-Fantini, Boyuan Pang, Sean G Byars, Regina B Oakley, Elizabeth J Perkins, Peter A Dargaville, Peter G Davis, Shuai Nie, Nicholas A Williamson, Vera Ignjatovic, David G Tingay
American Journal of Respiratory Cell and Molecular Biology | AMER THORACIC SOC | Published : 2019
The development of regional lung injury in the preterm lung is not well understood. This study aimed to characterize time-dependent and regionally specific injury patterns associated with early ventilation of the preterm lung using a mass spectrometry-based proteomic approach. Preterm lambs delivered at 124-127 days gestation received 15 or 90 minutes of mechanical ventilation (positive end-expiratory pressure = 8 cm H2O, Vt = 6-8 ml/kg) and were compared with unventilated control lambs. At study completion, lung tissue was taken from standardized nondependent and dependent regions, and assessed for lung injury via histology, quantitative PCR, and proteomic analysis using Orbitrap-mass spect..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) Centre of Research in Excellence research grant
Awarded by NHMRC Clinical Career Development Fellowship
Supported by Victorian Government's Operational Infrastructure Program funds to Murdoch Childrens Research Institute, and, in part, by National Health and Medical Research Council (NHMRC) Centre of Research in Excellence research grant 1057514 and project grant 1009287, and by NHMRC Clinical Career Development Fellowship grants 1053889 and 1123859 (D.G.T.).