Journal article

BCL-XL and MCL-1 are the key BCL-2 family proteins in melanoma cell survival

Erinna F Lee, Tiffany J Harris, Sharon Tran, Marco Evangelista, Surein Arulananda, Thomas John, Celeste Ramnac, Chloe Hobbs, Haoran Zhu, Gency Gunasingh, David Segal, Andreas Behren, Jonathan Cebon, Alexander Dobrovic, John M Mariadason, Andreas Strasser, Leona Rohrbeck, Nikolas K Haass, Marco J Herold, W Douglas Fairlie



Malignant melanoma is one of the most difficult cancers to treat due to its resistance to chemotherapy. Despite recent successes with BRAF inhibitors and immune checkpoint inhibitors, many patients do not respond or become resistant to these drugs. Hence, alternative treatments are still required. Due to the importance of the BCL-2-regulated apoptosis pathway in cancer development and drug resistance, it is of interest to establish which proteins are most important for melanoma cell survival, though the outcomes of previous studies have been conflicting. To conclusively address this question, we tested a panel of established and early passage patient-derived cell lines against several BH3-mi..

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Awarded by Worldwide Cancer Research Grant

Awarded by Australian Research Council Future Fellowship

Awarded by NHMRC

Awarded by Victorian Cancer Agency

Funding Acknowledgements

Support for this work was provided by a Worldwide Cancer Research Grant (#15-0025 to WDF, MJH, EFL, AS), an Australian Research Council Future Fellowship FT150100212 (to EFL), NHMRC Program Grant (#1016701 to AS), NHMRC Project Grants (#'s 1145728, 1143105 to MJH), and NHMRC Senior Principal Research Fellowship (#1020363 to AS), bequests from the Anthony Redstone Estate and Craig Perkins Cancer Research Foundation and a grant from the Victorian Cancer Agency (TRP13041 to WDF, MJH, EFL, AS). AB is the recipient of a Fellowship from the Victorian Government Department of Health and Human Services acting through the Victorian Cancer Agency. This work was made possible through Victoria State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme. We thank Grant McArthur, Karen Sheppard (Peter MacCallum Cancer Centre), and David Huang (Walter and Eliza Hall Institute of Medical Research) for the established melanoma cell lines used in this study and David Baloyan (Olivia Newton-John Cancer Research Institute) for flow cytometric expertise.