A New Subclade of Leptosphaeria biglobosa Identified from Brassica rapa
Zhongwei Zou, Xuehua Zhang, Paula Parks, Lindsey J du Toit, Angela P Van de Wouw, WG Dilantha Fernando
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | MDPI | Published : 2019
Blackleg (Phoma stem canker) of crucifers is a globally important disease caused by the ascomycete species complex comprising of Leptosphaeria maculans and Leptosphaeria biglobosa. Six blackleg isolates recovered from Brassica rapa cv. Mizspoona in the Willamette Valley of Oregon were characterized as L. biglobosa based on standard pathogenicity tests and molecular phylogenetic analysis. These isolates were compared to 88 characterized L. biglobosa isolates from western Canada, 22 isolates from Australia, and 6 L. maculans isolates from Idaho, USA using maximum parsimony and distance analysis of phylogenetic trees generated from the ITS rDNA (internal transcribed spacer rDNA) sequence, and t..View full abstract
This work was funded by the Natural Sciences and Engineering Research Council of Canada Discovery Program, and The Growing Forward 2 canola cluster funds awarded to W.G.D.F. through SaskCanola Agri-Science Project ASP program. The authors wish to thank Grains Research and Development Corporation for funding the Australian part of this study, and Washington State University and the Alfred Christianson Endowment for funding the Washington State component of the work.