Weevil pgrp-lb prevents endosymbiont TCT dissemination and chronic host systemic immune activation
Justin Maire, Carole Vincent-Monegat, Severine Balmand, Agnes Vallier, Mireille Herve, Florent Masson, Nicolas Parisot, Aurelien Vigneron, Caroline Anselme, Jackie Perrin, Julien Orlans, Isabelle Rahioui, Pedro Da Silva, Marie-Odile Fauvarque, Dominique Mengin-Lecreulx, Anna Zaidman-Remy, Abdelaziz Heddi
Proceedings of the National Academy of Sciences of USA | NATL ACAD SCIENCES | Published : 2019
Awarded by French (IMetSym)
Awarded by French (GREEN)
We thank C. Fiol, S. Lopez-Madrigal, and C. Vargas-Chavez for their valuable help on animal rearing and sampling; R. Rebollo for her critical reading of the manuscript; S. Hughes and B. Gillet (Institut de Genomique Fonctionnelle de Lyon) for RNA sequencing; N. Silverman (University of Massachusetts) for the fruitful discussion on novel ways to detect tracheal cytotoxin; B.-H. Oh (Korea Advanced Institute of Science and Technology) for providing Drosophila's pgrp-le sequence; T. Touze (Universite Paris Sud) for trying to produce recombinant PGRP-LBt; A. Khila's team (Institut de Genomique Fonctionnelle de Lyon) for providing Drosophila S2 cells; J.-Y. Rasplus for collecting Sitophilus linearis individuals from Niger; and G. Gabant and V. Aucagne (Center for Molecular Biophysics, Orleans) for mass spectrometry experiments. qPCR was performed at Developpement de Techniques et Analyse Moleculaire de la Biodiversite, from the Bio Environnement et Sante Research Federation. Confocal microscopy was performed at the Centre Technologique des Microstructures. Funding for this project was provided by Institut National de la Recherche Agronomique, Institut National des Sciences Appliquees-Lyon, the French ANR-13-BSV7-0016-01 (IMetSym), the French ANR-17-CE20-0031-01 (GREEN), and a grant from La Region Rhone-Alpes.