Journal article

The importance of the leader sequence for directing lanthionine formation in lacticin 481

Gregory C Patton, Moushumi Paul, Lisa E Cooper, Champak Chatterjee, Wilfred A van der Donk

BIOCHEMISTRY | AMER CHEMICAL SOC | Published : 2008

Abstract

Lantibiotics are post-translationally modified peptide antimicrobial agents that are synthesized with an N-terminal leader sequence and a C-terminal propeptide. Their maturation involves enzymatic dehydration of Ser and Thr residues in the precursor peptide to generate unsaturated amino acids, which react intramolecularly with nearby cysteines to form cyclic thioethers termed lanthionines and methyllanthionines. The role of the leader peptide in lantibiotic biosynthesis has been subject to much speculation. In this study, mutations of conserved residues in the leader sequence of the precursor peptide for lacticin 481 (LctA) did not inhibit dehydration and cyclization by lacticin 481 syntheta..

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