Journal article
Proteomic analysis of GLUT4 storage vesicles reveals tumor suppressor candidate 5 (TUSC5) as a novel regulator of insulin action in adipocytes
DJ Fazakerley, S Naghiloo, R Chaudhuri, F Koumanov, JG Burchfield, KC Thomas, JR Krycer, MJ Prior, BL Parker, BA Murrow, J Stöckli, CC Meoli, GD Holman, DE James
Journal of Biological Chemistry | ELSEVIER | Published : 2015
Abstract
Insulin signaling augments glucose transport by regulating glucose transporter 4 (GLUT4) trafficking from specialized intracellular compartments, termed GLUT4 storage vesicles (GSVs), to the plasma membrane. Proteomic analysis of GSVs by mass spectrometry revealed enrichment of 59 proteins in these vesicles. We measured reduced abundance of 23 of these proteins following insulin stimulation and assigned these as high confidence GSV proteins. These included established GSV proteins such as GLUT4 and insulin-responsive aminopeptidase, as well as six proteins not previously reported to be localized to GSVs. Tumor suppressor candidate 5 (TUSC5) was shown to be a novel GSV protein that underwent ..
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Awarded by Medical Research Council
Funding Acknowledgements
This work was supported in part by National Health and Medical Research Council of Australia Project Grant 1052782 (to D.J.F.). The authors declare that they have no conflicts of interest with the contents of this article. Supported by Medical Research Council UK and Diabetes UK. Supported by National Health and Medical Research Council of Australia Early Career Fellowships.