Cis- and trans-acting variants contribute to survivorship in a naive Drosophila melanogaster population exposed to ryanoid insecticides
Llewellyn Green, Paul Battlay, Alexandre Fournier-Level, Robert T Good, Charles Robin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2019
Insecticide resistance is a paradigm of microevolution, and insecticides are responsible for the strongest cases of recent selection in the genome of Drosophila melanogaster Here we use a naïve population and a novel insecticide class to examine the ab initio genetic architecture of a potential selective response. Genome-wide association studies (GWAS) of chlorantraniliprole susceptibility reveal variation in a gene of major effect, Stretchin Myosin light chain kinase (Strn-Mlck), which we validate with linkage mapping and transgenic manipulation of gene expression. We propose that allelic variation in Strn-Mlck alters sensitivity to the calcium depletion attributable to chlorantraniliprole'..View full abstract
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Awarded by Australia Research Council
We thank Trudy Mackay for advice and wisdom; Stephen Wilcox for his assistance with molecular biology analyses; Phil Batterham for UAS lines and discussion; Josefa Gonzalez for discussion; and John McKenzie for comments on the manuscript. This work was partially funded by the Australia Research Council Grant DP0985013.