Journal article
Changes in the leukocyte methylome and its effect on cardiovascular-related genes after exercise
J Denham, BJ O'Brien, FZ Marques, FJ Charchar
Journal of Applied Physiology | AMER PHYSIOLOGICAL SOC | Published : 2015
Abstract
Physical exercise has proven cardiovascular benefits, yet there is no clear understanding of the related molecular mechanisms leading to this. Here we determined the beneficial epigenetic effects of exercise after sprint interval training, a form of exercise known to improve cardiometabolic health. We quantified genome-wide leukocyte DNA methylation of 12 healthy young (18-24 yr) men before and after 4 wk (thrice weekly) of sprint interval training using the 450K BeadChip (Illumina) and validated gene expression changes in an extra seven subjects. Exercise increased subjects' cardiorespiratory fitness and maximal running performance, and decreased low-density lipoprotein cholesterol concentr..
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Funding Acknowledgements
Support for this study was provided by the Federation University Australia Self-sustaining Regions Research and Innovation Initiative, an Australian Government Collaborative Research Network. J. Denham is supported by an Australian Postgraduate Award scholarship. F. Z. Marques is supported by National Health and Medical Research Council (NHRMC) Grant APP1052659 and National Heart Foundation Grant PF12M6785 co-shared Early Career Fellowships. F. J. Charchar is supported by the Lew Carty Charitable Fund and NHRMC.