Journal article

Comparative and functional analyses of LYL1 loci establish marsupial sequences as a model for phylogenetic footprinting

MA Chapman, FJ Charchar, S Kinston, CP Bird, D Grafham, J Rogers, F Grutzner, JAM Graves, AR Green, B Gottgens

GENOMICS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2003

Abstract

Comparative genomic sequence analysis is a powerful technique for identifying regulatory regions in genomic DNA. However, its utility largely depends on the evolutionary distances between the species involved. Here we describe the screening of a genomic BAC library from the stripe-faced dunnart, Sminthopsis macroura, formerly known as the narrow-footed marsupial mouse. We isolated a clone containing the LYL1 locus, completely sequenced the 60.6-kb insert, and compared it with orthologous human and mouse sequences. Noncoding homology was substantially reduced in the human/dunnart analysis compared with human/mouse, yet we could readily identify all promoters and exons. Human/mouse/dunnart ali..

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University of Melbourne Researchers