Journal article

Absence of the beta 1 subunit of AMP-activated protein kinase reduces myofibroblast infiltration of the kidneys in early diabetes

Suet-Wan Choy, Scott A Fraser, Marina Katerelos, Sandra Galic, Bruce E Kemp, Peter F Mount, David A Power

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | WILEY | Published : 2019

Abstract

Activation of the heterotrimeric energy-sensing kinase AMP-activated protein kinase (AMPK) has been reported to improve experimental diabetic kidney disease. We examined the effect of type 1 diabetes in wild-type (WT) mice and mice lacking the β1 subunit of AMPK (AMPK β1-/- mice), which have reduced AMPK activity in kidneys and other organs. Diabetes was induced using streptozotocin (STZ) and the animals followed up for 4 weeks. Hyperglycaemia was more severe in diabetic AMPK β1-/- mice, despite the absence of any difference in serum levels of insulin, adiponectin and leptin. There was no change in AMPK activity in the kidneys of diabetic WT mice by AMPK activity assay, or phosphorylation of..

View full abstract