Recapitulating Parkinson's disease pathology in a three-dimensional human neural cell culture model.
Teresa R Taylor-Whiteley, Christine L Le Maitre, James A Duce, Caroline F Dalton, David P Smith
Dis Model Mech | Published : 2019
Extensive loss of dopaminergic neurons and aggregation of the protein α-synuclein into ubiquitin-positive Lewy bodies represents a major neuropathological hallmark of Parkinson's disease (PD). At present, the generation of large nuclear-associated Lewy bodies from endogenous wild-type α-synuclein, translationally regulated under its own promoter in human cell culture models, requires costly and time-consuming protocols. Here, we demonstrate that fully differentiated human SH-SY5Y neuroblastoma cells grown in three-dimensional cell culture develop Lewy-body-like pathology upon exposure to exogenous α-synuclein species. In contrast to most cell- and rodent-based PD models, which exhibit multip..View full abstract