High throughput LC-MS/MS-based proteomic analysis of excretory-secretory products from short-term in vitro culture of Haemonchus contortus
Tao Wang, Guangxu Ma, Ching-Seng Ang, Pasi K Korhonen, Anson Koehler, Neil D Young, Shuai Nie, Nicholas A Williamson, Robin B Gasser
Journal of Proteomics | ELSEVIER SCIENCE BV | Published : 2019
Parasitic nematodes of humans, animals and plants have a major, adverse impact on global health and agricultural production worldwide. To cope with their surrounding environment in and the immune attack from the host, excretory-secretory (ES) proteins are released by nematodes to orchestrate or regulate parasite-host interactions. In the present study, we characterised the ES products from short-term (12 h) in vitro culture of different developmental stages/sexes of Haemonchus contortus (one of the most important parasitic nematodes of livestock animals worldwide) using a high throughput tandem mass-spectrometry, underpinned by the most recent genomic dataset. In total, 878 unique proteins f..View full abstract
Funding from the National Health and Medical Research Council (NHMRC) of Australia, the Australian Research Council, Melbourne Water Corporation and The University of Melbourne (BIP) is gratefully acknowledged (R.B.G. et al.) as is support from the Melbourne Bioinformatics Platform. P.K.K. holds an NHMRC Early Career Researcher Fellowship (ECRF). N.D.Y. holds an NHMRC Career Development Fellowship (CDF1). Funding bodies played no role in the design of the study or collection, analysis or interpretation of data, or in the writing of the manuscript.