Journal article

Metabolomic and Transcriptional Analyses Reveal Atmospheric Oxygen During Human Induced Pluripotent Stem Cell Generation Impairs Metabolic Reprogramming

James Spyrou, David K Gardner, Alexandra J Harvey

STEM CELLS | WILEY | Published : 2019

Abstract

The transition to pluripotency invokes profound metabolic restructuring; however, reprogramming is accompanied by the retention of somatic cell metabolic and epigenetic memory. Modulation of metabolism during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how metabolite availability during reprogramming affects the physiology of resultant induced pluripotent stem cells (iPSCs). Metabolic analyses of iPSCs generated under either physiological (5%; P-iPSC) or atmospheric (20%; A-iPSC) oxygen conditions revealed that they retained aspects of somatic cell metabolic memory and failed to regulate carbohydrate metabolism with A-iPSC acquiring different metabol..

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Grants

Awarded by Australian Research Council Special Research Initiative: Stem Cells Australia


Funding Acknowledgements

We thank James G. Ryall at The University of Melbourne, Department of Physiology for his assistance in running the bioenergetic profiling assay and the Australian Genome Research Facility (AGRF) for their assistance in sample sequencing for RNA-seq analysis. This work was supported by Melbourne Research Fellowships (Career Interruptions) Grant to A.J.H. and the Australian Research Council Special Research Initiative: Stem Cells Australia (SR1101002) to D.K.G.