Metabolomic and Transcriptional Analyses Reveal Atmospheric Oxygen During Human Induced Pluripotent Stem Cell Generation Impairs Metabolic Reprogramming
James Spyrou, David K Gardner, Alexandra J Harvey
STEM CELLS | WILEY | Published : 2019
The transition to pluripotency invokes profound metabolic restructuring; however, reprogramming is accompanied by the retention of somatic cell metabolic and epigenetic memory. Modulation of metabolism during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how metabolite availability during reprogramming affects the physiology of resultant induced pluripotent stem cells (iPSCs). Metabolic analyses of iPSCs generated under either physiological (5%; P-iPSC) or atmospheric (20%; A-iPSC) oxygen conditions revealed that they retained aspects of somatic cell metabolic memory and failed to regulate carbohydrate metabolism with A-iPSC acquiring different metabol..View full abstract
Awarded by Australian Research Council Special Research Initiative: Stem Cells Australia
We thank James G. Ryall at The University of Melbourne, Department of Physiology for his assistance in running the bioenergetic profiling assay and the Australian Genome Research Facility (AGRF) for their assistance in sample sequencing for RNA-seq analysis. This work was supported by Melbourne Research Fellowships (Career Interruptions) Grant to A.J.H. and the Australian Research Council Special Research Initiative: Stem Cells Australia (SR1101002) to D.K.G.