Journal article

Supramolecular Platform Stabilizing Growth Factors

Simone IS Hendrikse, Sergio Spaans, EW Meijer, Patricia YW Dankers

Biomacromolecules | AMER CHEMICAL SOC | Published : 2018

Abstract

High concentrations of supplemented growth factors can cause oversaturation and adverse effects in in vitro and in vivo studies, though these supraphysiological concentrations are often required due to the low stability of growth factors. Here we demonstrate the stabilization of TGF-β1 and BMP4 using supramolecular polymers. Inspired by heparan sulfate, sulfonated peptides were presented on a supramolecular polymer to allow for noncovalent binding to growth factors in solution. After mixing with excipient molecules, both TGF-β1 and BMP4 were shown to have a prolonged half-life compared to the growth factors free in solution. Moreover, high cellular response was measured by a luciferase assay..

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University of Melbourne Researchers

Grants

Awarded by NWO/DPI program NEWPOL


Awarded by Ministry of Education, Culture and Science


Awarded by European Research Council (FP7) ERC Grant


Awarded by Netherlands Cardiovascular Research Initiative


Funding Acknowledgements

This work was funded by the NWO/DPI program NEWPOL (Project No. 731.015.503), partially financed with TKI (Topconsortia for Knowledge Innovation) allowance provided by the Dutch Ministry of Economic Affairs. E.W.M. acknowledges the Ministry of Education, Culture and Science (Gravity program FMS, 024.001.035). P.Y.W.D. acknowledges the European Research Council (FP7/2007-2013) ERC Grant Agreement 308045. We also acknowledge the support from The Netherlands Cardiovascular Research Initiative (CVON 2012-01), the Dutch Heart Foundation, Dutch Federation of University Medical Centers, The Netherlands Organization for Health Research and Development, and the Royal Netherlands Academy of Sciences. The authors thank Prof. Marie Jose Goumans (Leiden University Medical Center, The Netherlands) for providing the HT1080 cells.