Journal article

Effect of NADPH oxidase 1 and 4 blockade in activated human retinal endothelial cells

Binoy Appukuttan, Yuefang Ma, Andrew Stempel, Liam M Ashander, Devy Deliyanti, Jennifer L Wilkinson-Berka, Justine R Smith

CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | WILEY | Published : 2018

Abstract

BACKGROUND: Over-production of reactive oxygen species (ROS) and resulting oxidative stress contribute to retinal damage in vascular diseases that include diabetic retinopathy, retinopathy of prematurity and major retinal vessel occlusions. NADPH oxidase (Nox) proteins are professional ROS-generating enzymes, and therapeutic targeting in these diseases has strong appeal. Pharmacological inhibition of Nox4 reduces the severity of experimental retinal vasculopathy. We investigated the potential application of this drug approach in humans. METHODS: Differential Nox enzyme expression was studied by real-time-quantitative polymerase chain reaction in primary human retinal endothelial cell isolate..

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