Localization studies of IGFBP-2 and IGFBP-5 in the anterior compartment of the eye

Abstract

Purpose. Insulin-like growth factor binding proteins (IGFBPs) may modulate insulin-like growth factor-I (IGF-I) action and are important regulating factors in the IGF system. Our aim was to determine the presence of IGFBP-2 and -5 in the anterior compartment of the eye and to compare the histological sites of these IGFBP proteins with the respective IGFBP mRNAs. Methods. To investigate this, immunohistochemistry was used to detect the presence of IGFBP-2 and -5 proteins, and in situ hybridization was used to determine the presence of IGFBP-2 and -5 mRNAs. The studies were performed in normal adult male Sprague-Dawley rats. Immunohistochemistry was per formed by the immunoperoxidase method wi..