Journal article

Mesenchyme to epithelial transition protein expression, gene copy number and clinical outcome in a large non-small cell lung cancer surgical cohort

Gareth Rivalland, Paul Mitchell, Carmel Murone, Khashayer Asadi, Adrienne L Morey, Maud Starmans, Paul C Boutros, Marzena Walkiewicz, Benjamin Solomon, Gavin Wright, Simon Knight, Thomas John

TRANSLATIONAL LUNG CANCER RESEARCH | AME PUBL CO | Published : 2019

Abstract

Background: In non-small cell lung cancer (NSCLC), mesenchyme to epithelial transition (MET) protein abundance increases with disease stage and is implicated in resistance to tyrosine kinase inhibitors. To better clarify the impact of MET overexpression on tumor behavior, we investigated a large cohort of patients who underwent curative surgical resection to determine whether MET gene amplification or protein abundance was prognostic. Methods: Tissue microarrays (TMAs) were constructed using triplicate 1 mm cores of FFPE primary NSCLC specimens. TMAs underwent immunohistochemical (IHC) staining with the SP44 clone (Ventana) and cores were considered positive if >50% of tumor exhibited 2+ sta..

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