Journal article

Endogenous Glutamate Excites Myenteric Calbindin Neurons by Activating Group I Metabotropic Glutamate Receptors in the Mouse Colon

Mathusi Swaminathan, Elisa L Hill-Yardin, Joel C Bornstein, Jaime PP Foong

FRONTIERS IN NEUROSCIENCE | FRONTIERS MEDIA SA | Published : 2019

Abstract

Glutamate is a classic excitatory neurotransmitter in the central nervous system (CNS), but despite several studies reporting the expression of glutamate together with its various receptors and transporters within the enteric nervous system (ENS), its role in the gut remains elusive. In this study, we characterized the expression of the vesicular glutamate transporter, vGluT2, and examined the function of glutamate in the myenteric plexus of the distal colon by employing calcium (Ca2+)-imaging on Wnt1-Cre; R26R-GCaMP3 mice which express a genetically encoded fluorescent Ca2+ indicator in all enteric neurons and glia. Most vGluT2 labeled varicosities contained the synaptic vesicle release pro..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Research Council


Funding Acknowledgements

This research was supported by National Health and Medical Research Council of Australia Project grants #1099016 (JF and JB), and Australian Research Council grant #DP130101596 (JB).