Journal article
Genomic DNA methylation distinguishes subtypes of human focal cortical dysplasia
K Kobow, M Ziemann, H Kaipananickal, I Khurana, A Mühlebner, M Feucht, JA Hainfellner, T Czech, E Aronica, T Pieper, H Holthausen, M Kudernatsch, H Hamer, BS Kasper, K Rössler, V Conti, R Guerrini, R Coras, I Blümcke, A El-Osta Show all
Epilepsia | WILEY | Published : 2019
DOI: 10.1111/epi.14934
Abstract
Objectives: Focal cortical dysplasia (FCD) is a major cause of drug-resistant focal epilepsy in children, and the clinicopathological classification remains a challenging issue in daily practice. With the recent progress in DNA methylation–based classification of human brain tumors we examined whether genomic DNA methylation and gene expression analysis can be used to also distinguish human FCD subtypes. Methods: DNA methylomes and transcriptomes were generated from massive parallel sequencing in 15 surgical FCD specimens, matched with 5 epilepsy and 6 nonepilepsy controls. Results: Differential hierarchical cluster analysis of DNA methylation distinguished major FCD subtypes (ie, Ia, IIa, a..
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Awarded by Seventh Framework Programme
Funding Acknowledgements
FP7 Health, Grant/Award Number: GA602531; ZonMw, Grant/Award Number: 95105004; National Health and Medical Research Council, Grant/Award Number: APP1075563