Induction of sodium iodide symporter gene and molecular characterisation of HNF3 beta/FoxA2, TTF-1 and C/EBP beta in thyroid carcinoma cells
T Akagi, QT Luong, D Gui, J Said, J Selektar, A Yung, CM Bunce, GD Braunstein, HP Koeffler
BRITISH JOURNAL OF CANCER | NATURE PUBLISHING GROUP | Published : 2008
Thyroid carcinoma cells often do not express thyroid-specific genes including sodium iodide symporter (NIS), thyroperoxidase (TPO), thyroglobulin (TG), and thyrotropin-stimulating hormone receptor (TSHR). Treatment of thyroid carcinoma cells (four papillary and two anaplastic cell lines) with histone deacetylase inhibitors (SAHA or VPA) modestly induced the expression of the NIS gene. The promoter regions of the thyroid-specific genes contained binding sites for hepatocyte nuclear factor 3 beta (HNF3 beta)/forkhead box A2 (FoxA2), thyroid transcription factor 1 (TTF-1), and CCAAT/enhancer binding protein (C/EBP beta). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) show..View full abstract
We are grateful to Drs Shlomo Melmed and Edward Morrisey for gifts of FRTL-5 cell lines and the pTTF-1 and pHNF3 beta/FoxA2 plasmids, respectively. We thank Drs Adrian F Gombart and Sigal Gery for helpful discussions. We acknowledge the generous support of the Inger Foundation, and the C & H Koeffler Fund. GDB is holder of the James R Klinenberg, MD, Chair in Medicine. HPK is the holder of the Mark Goodson Chair in Oncology Research at Cedars-Sinai Medical Center, and is a member of the Jonsson Cancer Center and the Molecular Biology Institute at UCLA.