Journal article

Quantifying NK cell growth and survival changes in response to cytokines and regulatory checkpoint blockade helps identify optimal culture and expansion conditions

Robert J Hennessy, Kim Pham, Rebecca Delconte, Jai Rautela, Philip D Hodgkin, Nicholas D Huntington

JOURNAL OF LEUKOCYTE BIOLOGY | WILEY | Published : 2019

Abstract

NK cells are innate lymphocytes critical for immune surveillance, particularly in eradication of metastatic cancer cells and acute antiviral responses. In contrast to T cells, NK cell-mediated immunity is rapid, with spontaneous cytotoxicity and cytokine/chemokine production upon pathogen detection. The renaissance in cancer immunology has cast NK cell biology back into the spotlight with an urgent need for deeper understanding of the regulatory networks that govern NK cell antitumor activity. To this end, we have adapted and refined a series of quantitative cellular calculus methods, previously applied to T and B lymphocytes, to dissect the biologic outcomes of NK cells following stimulatio..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by Melanoma Research Grant from the Harry J Lloyd Charitable Trust


Awarded by Melanoma Research Alliance Young Investigator Award


Awarded by CLIP grant from Cancer Research Institute


Funding Acknowledgements

This work was supported by project grants from the National Health and Medical Research Council (NHMRC) of Australia (#1124784, #1066770, #1057852, #1124907 to N.D.H.; and #1010654 to P.D.H.). N.D.H. is a NHMRC CDF2 Fellow (1124788), a recipient of a Melanoma Research Grant from the Harry J Lloyd Charitable Trust, Melanoma Research Alliance Young Investigator Award, and a CLIP grant from Cancer Research Institute. P.D.H. is a NHMRC PRF Fellow National Health. This study was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC Independent Research Institute Infrastructure Support scheme.