Overexpression of a Monocot Acyl-CoA-Binding Protein Confers Broad-Spectrum Pathogen Protection in a Dicot
Saritha Panthapulakkal Narayanan, Pan Liao, Paul WJ Taylor, Clive Lo, Mee-Len Chye
PROTEOMICS | WILEY | Published : 2019
Plants are continuously infected by various pathogens throughout their lifecycle. Previous studies have reported that the expression of Class III acyl-CoA-binding proteins (ACBPs) such as the Arabidopsis ACBP3 and rice ACBP5 were induced by pathogen infection. Transgenic Arabidopsis AtACBP3-overexpressors (AtACBP3-OEs) displayed enhanced protection against the bacterial biotroph, Pseudomonas syringae, although they became susceptible to the fungal necrotroph Botrytis cinerea. A Class III ACBP from a monocot, rice (Oryza sativa) OsACBP5 was overexpressed in the dicot Arabidopsis. The resultant transgenic Arabidopsis lines conferred resistance not only to the bacterial biotroph P. syringae but..View full abstract
Awarded by Research Grants Council of Hong Kong Special Administrative Region, China
S.P.N. performed most of the experiments. S.P.N and P.L. analyzed proteomic data. S.P.N., P.L., P.T., C.L., and M.L.C. analyzed data. S.P.N. and M.L.C. designed the research and wrote the manuscript with contribution from all authors. The authors thank Dr. Rumiana Ray (University of Nottingham) for the fungal strain R. solani AG-1-1 (ATCC 66157). This work was supported by the Wilson and Amelia Wong Endowment Fund and the Research Grants Council of Hong Kong Special Administrative Region, China (17109917, AoE/M-403/16 and AoE/M-05/12) to M.L.C. S.P.N. was supported by an HKU Postgraduate Studentship, and P.L. by AoE/M-05/12.