Journal article

Expression and localization of heat-shock proteins during skeletal muscle cell proliferation and differentiation and the impact of heat stress

Savant S Thakur, Janine L James, Nicola J Cranna, Victoria L Chhen, Kristy Swiderski, James G Ryall, Gordon S Lynch



Skeletal myogenesis is a coordinated sequence of events associated with dramatic changes in cell morphology, motility, and metabolism, which causes cellular stress and alters proteostasis. Chaperones, such as heat-shock proteins (HSPs), play important roles in limiting cellular stresses and maintaining proteostasis, but whether HSPs are specifically involved in myogenesis is not well understood. Here, we characterized gene and protein expression and subcellular localization of various HSPs in proliferating C2C12 myoblasts and differentiating myotubes under control conditions and in response to heat stress. Hsp25, Hsp40, and Hsp60 protein expression declined by 48, 35, and 83%, respectively, ..

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Awarded by National Health and Medical Research Council of Australia (NHMRC)

Awarded by Muscular Dystrophy Association, USA

Funding Acknowledgements

This project was supported by grants from the National Health and Medical Research Council of Australia (NHMRC; GNT1065456) and the Muscular Dystrophy Association, USA (MDA255153). SST is supported by the Research Training Program Scholarship, the June Opie Fellowship, and the Lionel Murphy Endowment Scholarship. KS and JGR were supported by the Early Career Fellowships from the NHMRC