Journal article

Quantification of Cytoskeletal Dynamics in Time-Lapse Recordings.

René Schneider, Arun Sampathkumar, Staffan Persson

Curr Protoc Plant Biol | Published : 2019

Abstract

The cytoskeleton is key to many essential processes in a plant cell, e.g., growth, division, and defense. Contrary to what "skeleton" implies, the cytoskeleton is highly dynamic, and is able to re-organize itself continuously. The advent of live-cell microscopy and the development of genetically encoded fluorophores enabled detailed observation of the organization and dynamics of the cytoskeleton. Despite the biological importance of the cytoskeletal dynamics, quantitative analyses remain laborious endeavors that only a handful of research teams regularly conduct. With this protocol, we provide a standardized step-by-step guide to analyze the dynamics of microtubules. We provide example data..

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University of Melbourne Researchers