Journal article

Molecular Classification of Barley (Hordeum vulgare L.) Mutants Using Derivative NIR Spectroscopy

Paul R Wiley, Greg J Tanner, Peter M Chandler, Robert S Anderssen

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | AMER CHEMICAL SOC | Published : 2009

Abstract

Near-infrared reflectance (NIR) spectroscopy was used in the characterization of grain morphology mutants of barley ( Hordeum vulgare L.) in relation to grain nitrogen (N) content and protein composition. Derivative spectroscopy provided spectra with enhanced resolution, allowing wavelengths to be identified with clear differences in contribution from associated chemical bonds. Comparisons of fourth-derivative spectra of wholemeal flour from high-N grains with flour from low-N grains identified wavelengths at which there were statistically significant differences between the groups. Their importance was independently confirmed by step-up regression using these wavelengths to generate an equa..

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University of Melbourne Researchers