Journal article

Proanthocyanidin synthesis in the forage legume Onobrychis viciifolia. A study of chalcone synthase, dihydroflavonol 4-reductase and leucoanthocyanidin 4-reductase in developing leaves

R Joseph, G Tanner, P Larkin

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY | C S I R O PUBLICATIONS | Published : 1998

Abstract

The timing of proanthocyanidin (PA) biosynthesis in leaves of Onobrychis viciifolia (sainfoin) was examined using nucleotide probes, enzyme assays and immunochemical detection. To achieve this, a chalcone synthase (CHS) cDNA clone was isolated and a PCR-amplified DNA fragment corresponding to part of a dihydroflavonol reductase (DFR) gene was obtained from sainfoin. The high sequence homology of the CHS clone, both at the nucleotide and amino acid levels, with CHS sequences from other species, confirmed the identity of the clone. The identity of the PCR fragment was confirmed by sequence homology to known DFR sequences. The level of DFR mRNA, DFR and leucoanthocyanidin reductase (LAR) enzyme..

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