Quantification of Hordeins by ELISA: The Correct Standard Makes a Magnitude of Difference
Gregory J Tanner, Malcolm J Blundell, Michelle L Colgrave, Crispin A Howitt
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013
BACKGROUND: Coeliacs require a life-long gluten-free diet supported by accurate measurement of gluten (hordein) in gluten-free food. The gluten-free food industry, with a value in excess of $6 billion in 2011, currently depends on two ELISA protocols calibrated against standards that may not be representative of the sample being assayed. AIM: The factors affecting the accuracy of ELISA analysis of hordeins in beer were examined. RESULTS: A simple alcohol-dithiothreitol extraction protocol successfully extracts the majority of hordeins from barley flour and malt. Primary hordein standards were purified by FPLC. ELISA detected different classes of purified hordeins with vastly different se..View full abstract
Awarded by Grains Research and Development Commission (Australia)
This work was supported by grants CSP00051, CSP00067, CSP00088, and CSP00113 from the Grains Research and Development Commission (Australia), and appropriation funds from the Commonwealth Scientific and Industrial Research Organisation Food Futures National Research Flagship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.