Journal article

Lighting Up Mutation: a New Unbiased System for the Measurement of Microbial Mutation Rates

Kylie J Boyce, Alexander Idnurm

mBio | AMER SOC MICROBIOLOGY | Published : 2019

Abstract

Although mutation drives evolution over long and short terms, measuring and comparing mutation rates accurately have been particularly difficult. This is especially true when mutations lead to an alteration in fitness. E. Shor, J. Schuyler, and D. S. Perlin (https://doi.org/10.1128/mBio.00120-19) present a new method to compare mutation rates across fungal strains and under different growth conditions: they employ the green fluorescent protein (GFP) as the reporter and count mutations using fluorescence-activated cell sorting (FACS). The estimates of mutation rates using the GFP-FACS approach are similar to those calculated with other reporters, and the method was used to assess if different..

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University of Melbourne Researchers