Journal article

ROCK1 but not LIMK1 or PAK2 is a key regulator of apoptotic membrane blebbing and cell disassembly

Rochelle Tixeira, Kha Phan Thanh, Sarah Caruso, Bo Shi, Georgia K Atkin-Smith, Christina Nedeva, Jenny DY Chow, Hamsa Puthalakath, Mark D Hulett, Marco J Herold, Ivan KH Poon

CELL DEATH AND DIFFERENTIATION | NATURE PUBLISHING GROUP | Published : 2020

Abstract

Many cell types are known to undergo a series of morphological changes during the progression of apoptosis, leading to their disassembly into smaller membrane-bound vesicles known as apoptotic bodies (ApoBDs). In particular, the formation of circular bulges called membrane blebs on the surface of apoptotic cells is a key morphological step required for a number of cell types to generate ApoBDs. Although apoptotic membrane blebbing is thought to be regulated by kinases including ROCK1, PAK2 and LIMK1, it is unclear whether these kinases exhibit overlapping roles in the disassembly of apoptotic cells. Utilising both pharmacological and CRISPR/Cas9 gene editing based approaches, we identified R..

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University of Melbourne Researchers

Grants

Awarded by National Health & Medical Research Council of Australia


Awarded by Australian Research Council


Funding Acknowledgements

We would like to thank the LIMS BioImaging Facility for assistance with microscopy. We would also like to thank Alyce Mayfosh for assistance with quantitative PCR. This work was supported by grants from the National Health & Medical Research Council of Australia (GNT1125033 and GNT1140187) and Australian Research Council (DP170103790) to I.K.H.P.