Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk
Alexander N Combes, Belinda Phipson, Kynan T Lawlor, Aude Dorison, Ralph Patrick, Luke Zappia, Richard P Harvey, Alicia Oshlack, Melissa H Little
DEVELOPMENT | COMPANY BIOLOGISTS LTD | Published : 2019
Recent advances in the generation of kidney organoids and the culture of primary nephron progenitors from mouse and human have been based on knowledge of the molecular basis of kidney development in mice. Although gene expression during kidney development has been intensely investigated, single cell profiling provides new opportunities to further subsect component cell types and the signalling networks at play. Here, we describe the generation and analysis of 6732 single cell transcriptomes from the fetal mouse kidney [embryonic day (E)18.5] and 7853 sorted nephron progenitor cells (E14.5). These datasets provide improved resolution of cell types and specific markers, including subdivision o..View full abstract
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Awarded by Australian Research Council
Awarded by National Health and Medical Research Council (NHMRC)
This work was supported by seed funding from the Murdoch Children's Research Institute (which is supported by the State Government of Victoria's Operational Infrastructure Support Program) and the University of Melbourne, and grants from the Australian Research Council (DE150100652 to A.N.C.) and the National Health and Medical Research Council (NHMRC) (GNT1156567 to A.N.C., A.O., M.H.L.). M.H.L. is a Senior Principal Research Fellow of the NHMRC (GNT1136085). A.O. is a Career Development Fellow of the NHMRC (APP1126157). L.Z. is supported by a Department of Education and Training, Australian Government, Research Training Program (RTP) Scholarship.