Analysis of extracellular vesicles generated from monocytes under conditions of lytic cell death
Amy A Baxter, Thanh Kha Phan, Eric Hanssen, Michael Liem, Mark D Hulett, Suresh Mathivanan, Ivan KH Poon
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2019
Extracellular vesicles (EVs) are an important class of membrane-bound structures that have been widely investigated for their roles in intercellular communication in the contexts of tumor progression, vascular function, immunity and regenerative medicine. Much of the current knowledge on the functions of EVs pertains to those derived from viable cells (e.g. exosomes and microvesicles) or apoptotic cells (e.g. apoptotic bodies) whilst the generation of EVs from dying cells under non-apoptotic conditions remains poorly characterized. Herein, the release of EVs from THP-1 monocytes under conditions of primary necrosis, secondary necrosis and pyroptosis, was investigated. A comprehensive analysi..View full abstract
Awarded by Australian Research Council
Awarded by National Health & Medical Research Council of Australia
We would like to thank A/Prof Seth Masters (Walter and Eliza Hall Institute, Parkville, VIC, Australia) for his generous gifting of reagents, advice and discussions relating to inflammasome activation. We would like to thank Mitch Shambrook and members of the Hill laboratory (LIMS) for assistance using the NanoSight NS300 and A/Prof Adam Mechler (LIMS) for assistance with DLS. We would like to thank the LIMS BioImaging Facility for assistance with microscopy. This work was supported by grants from the Australian Research Council (DP170103790) and National Health & Medical Research Council of Australia (GNT1125033 and GNT1140187) to I.K.H.P., and National Health & Medical Research Council of Australia (GNT1141732) to A.A.B.