Organotypic Culture of Neonatal Murine Inner Ear Explants
Jacqueline M Ogier, Rachel A Burt, Hannah R Drury, Rebecca Lim, Bryony A Nayagam
FRONTIERS IN CELLULAR NEUROSCIENCE | FRONTIERS MEDIA SA | Published : 2019
The inner ear is a complex organ containing highly specialised cell types and structures that are critical for sensing sound and movement. In vivo, the inner ear is difficult to study due to the osseous nature of the otic capsule and its encapsulation within an intricate bony labyrinth. As such, mammalian inner ear explants are an invaluable tool for the study and manipulation of the complex intercellular connections, structures, and cell types within this specialised organ. The greatest strength of this technique is that the complete organ of Corti, or peripheral vestibular organs including hair cells, supporting cells and accompanying neurons, is maintained in its in situ form. The greates..View full abstract
Awarded by National Health and Medical Research Council of Australia (GNT)
This work was supported by the Garnet Passe and Rodney Williams Memorial Foundation (Ph.D. Research scholarship to JO; Research Fellowship to BN); the National Health and Medical Research Council of Australia (GNT#1023372); the Victorian State Government's Operational Infrastructure Support Program and the Australian Government's NHMRC IRIISS, UoN Research Fellowhip (RL).