Journal article

The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development

Lucas G Dent, Samuel A Manning, Benjamin Kroeger, Audrey M Williams, Abdul Jabbar Saiful Hilmi, Luke Crea, Shu Kondo, Sally Horne-Badovinac, Kieran F Harvey

PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2019

Abstract

How biochemical and mechanical information are integrated during tissue development is a central question in morphogenesis. In many biological systems, the PIX-GIT complex localises to focal adhesions and integrates both physical and chemical information. We used Drosophila melanogaster egg chamber formation to study the function of PIX and GIT orthologues (dPix and Git, respectively), and discovered a central role for this complex in controlling myosin activity and epithelial monolayering. We found that Git's focal adhesion targeting domain mediates basal localisation of this complex to filament structures and the leading edge of migrating cells. In the absence of dpix and git, tissue disru..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Australian Research Council


Awarded by National Institute of Health


Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

This work was supported in part by National Health and Medical Research Council (https://nhmrc.gov.au/) grant APP1020316 (KFH), by Australian Research Council (https://www.arc.gov.au) grant DP180102044 (KFH), and by National Institute of Health (https://www.nih.gov/) grants R01 GM126047 (SHB) and R01 GM126047 (AMW). KFH is a National Health and Medical Research Council Senior Research Fellow (Application ID, 1078220). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.