The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology
Vasanthakumar Ajithkumar, Ranjan Prasad
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | ELSEVIER | Published : 2010
Regulatory proteins that bind to upstream un-translated region often control transcription of prokaryotic genes. Many of these proteins bend or distort their DNA binding sites, and the induced DNA curvature facilitates protein-protein or protein-DNA contacts essential for transcriptional regulation. DnrO is an essential transcription regulator of Streptomyces peucetius that activates daunorubicin biosynthetic pathway. It binds to a specific sequence adjacent to dnrN promoter to activate transcription. The same binding event represses its own transcription. DNA binding domain of DnrO is within 60 aa from N-terminal end of the 340 aa protein. Helix-turn-helix motif in DnrO is similar to BirA r..View full abstract
Authors thank Department of Biotechnology, Government of India for financial support. Additional funds from UPE project of Madurai Kamaraj University (MKU), India supported by University Grants Commission, India is acknowledged. Authors thank Prof. K. Dharmalingam for his critical comments and technical support. Instrument support given by DBT Centre for Genetic Engineering and Strain Manipulation, at MKU is acknowledged.