A novel protein that binds to dnrN-dnrO intergenic region of Streptomyces peucetius purified by DNA affinity capture has dihydrolipoamide dehydrogenase activity
Vasantha Kumar Ajith, Ranjan Prasad
PROTEIN EXPRESSION AND PURIFICATION | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2009
An antitumour chemotherapeutic, daunorubicin (DNR), produced by Streptomyces peucetius exhibits cytotoxic activity through topoisomerase-mediated interaction with DNA, thereby inhibiting DNA replication and repair and RNA and protein synthesis. It is synthesized by the type II polyketide pathway. Understanding molecular mechanisms that drive expression of antibiotic biosynthetic genes in response to diverse signals and chemical inducers is of considerable interest. Intergenic DNA between regulatory genes dnrN and dnrO of DNR biosynthesis pathway in S. peucetius has a promoter for transcription of dnrN in one strand and three promoters in the opposite strand for dnrO. Studies have shown that ..View full abstract
Authors thank Department of Biotechnology, Government of India for financial support. Additional funds from UPE project of Madurai Kamaraj University (MKU), India supported by University Grants Commission, India is acknowledged. Authors thank Prof. K. Dharmalingam for his critical comments and technical support. Instrument support given by DBT Centre for Genetic Engineering and Strain Manipulation, at MKU is acknowledged. Proteomics central facility at School of Biotechnology, MKU is gratefully acknowledged.