Journal article

MicroRNA Biogenesis Machinery Is Dysregulated in the Endometrium of Infertile Women Suggesting a Role in Receptivity and Infertility

Hannah Lake, Kate Rainczuk, Evdokia Dimitriadis

Journal of Histochemistry & Cytochemistry | SAGE PUBLICATIONS LTD | Published : 2019


MicroRNAs (miRs) regulate endometrial function and their dysregulation could underlie unexplained infertility in women. Ribonucleases including DICER and DROSHA, and the proteins, ARGONAUTE 1 (AGO 1) and 2 (AGO 2) regulate the biogenesis/maturation of miRs. We aimed to elucidate the expression and localization of miR biogenesis machinery components during the human menstrual cycle and compare their levels in endometrium from women with normal fertility and primary unexplained infertility. miR biogenesis components were measured by quantitative-RT-PCR and immunohistochemistry. In the endometrium of women with normal fertility, DROSHA immunolocalized maximally to the epithelium during the earl..

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Funding Acknowledgements

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by funding for a project grant and senior research fellowship from the National Health and Medical Research Council (NHMRC) of Australia to ED. This work was supported by the Victorian Government's Operational Infrastructure Support Program.